Tesco is planning to open a 24,000 sw ft store in Stockton Street on a site currently occupied by Carlisle Interconnect Technologies.
Local residents and traders are concerned that it would damage the character of Littleborough. They are collecting signatures in a petition to stop the build of the store.
Littleborough is a town, which prides itself on local shops and local produce.
Campaigners are also worried about the traffic that would come into Littleborough, not only from the delivery trucks but also from the amount of people travelling to Littleborough to shop at the store.